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protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit  (Thermo Fisher)


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    Thermo Fisher protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit
    ( a ) Autoradiograph of protein <t>microarray</t> showing duplicate spots of recombinant ABLIM1 without (left) or with (right) recombinant DYRK1A, both with radioactive ATP. ( b ) Co-immunoprecipitation of exogenous DYRK1A and ABLIM1 proteins in Hek293T cells. Shown is a representative blot of three independent experiments. ( c ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM1. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( d ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM2. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( e ) Deconvolution images of F-actin fibres (phalloidin) in NIH3T3 cells transiently expressing the indicated constructs. Transfected cells were identified through co-transfection of nuclear H2B–GFP. The F-actin promoting Profilin (PFN1) was included as a positive control. Scale bar, 10 μm. ( f ) Quantification of the microscopy experiment depicted in e (mean of two independent experiments with at least 50 cells counted in each experiment). ARR, actin-rich region (lamellipodia, stress fibres, membrane ruffles or pronounced phalloidin positivity). ( g ) Subcellular localization of MKL1-GFP (green) in transiently transfected Hek293A cells. Representative pictures are shown. Nuclei appear in blue (4,6-diamidino-2-phenylindole). Scale bar, 10 μm. ( h ) Quantification of the experiment depicted in f . C, cytoplasmic; N, nuclear; N+C, nuclear and cytoplasmic localization of MKL1-GFP (mean of n =3 independent experiments. At least 100 cells were counted). ( i ) SRE (serum response element) luciferase reporter assay in Hek293T cells transiently transfected with the indicated constructs (mean of n =6±s.d.). ( j ) Phalloidin staining of siRNA-transfected NIH3T3 cells. Shown are representative images of two independent experiments. siABlim1/2: double knockdown of Ablim1 plus Ablim2 . Scale bar, 10 μm. ( k ) Phalloidin staining of siRNA/plasmid double-transfected NIH3T3 cells. The inset in the middle panel should help portrait the different phenotypes observed upon Ablim1 knockdown. siABlim1/2: double knockdown of Ablim1 plus Ablim2. Scale bar, 10 μm. * P <0.05; ** P <0.005; *** P <0.0005 (Student's t -test).
    Protoarray Human Protein Microarray V5.0 Kinase Substrate Identification (Ksi) Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Identification of a novel actin-dependent signal transducing module allows for the targeted degradation of GLI1"

    Article Title: Identification of a novel actin-dependent signal transducing module allows for the targeted degradation of GLI1

    Journal: Nature Communications

    doi: 10.1038/ncomms9023

    ( a ) Autoradiograph of protein microarray showing duplicate spots of recombinant ABLIM1 without (left) or with (right) recombinant DYRK1A, both with radioactive ATP. ( b ) Co-immunoprecipitation of exogenous DYRK1A and ABLIM1 proteins in Hek293T cells. Shown is a representative blot of three independent experiments. ( c ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM1. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( d ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM2. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( e ) Deconvolution images of F-actin fibres (phalloidin) in NIH3T3 cells transiently expressing the indicated constructs. Transfected cells were identified through co-transfection of nuclear H2B–GFP. The F-actin promoting Profilin (PFN1) was included as a positive control. Scale bar, 10 μm. ( f ) Quantification of the microscopy experiment depicted in e (mean of two independent experiments with at least 50 cells counted in each experiment). ARR, actin-rich region (lamellipodia, stress fibres, membrane ruffles or pronounced phalloidin positivity). ( g ) Subcellular localization of MKL1-GFP (green) in transiently transfected Hek293A cells. Representative pictures are shown. Nuclei appear in blue (4,6-diamidino-2-phenylindole). Scale bar, 10 μm. ( h ) Quantification of the experiment depicted in f . C, cytoplasmic; N, nuclear; N+C, nuclear and cytoplasmic localization of MKL1-GFP (mean of n =3 independent experiments. At least 100 cells were counted). ( i ) SRE (serum response element) luciferase reporter assay in Hek293T cells transiently transfected with the indicated constructs (mean of n =6±s.d.). ( j ) Phalloidin staining of siRNA-transfected NIH3T3 cells. Shown are representative images of two independent experiments. siABlim1/2: double knockdown of Ablim1 plus Ablim2 . Scale bar, 10 μm. ( k ) Phalloidin staining of siRNA/plasmid double-transfected NIH3T3 cells. The inset in the middle panel should help portrait the different phenotypes observed upon Ablim1 knockdown. siABlim1/2: double knockdown of Ablim1 plus Ablim2. Scale bar, 10 μm. * P <0.05; ** P <0.005; *** P <0.0005 (Student's t -test).
    Figure Legend Snippet: ( a ) Autoradiograph of protein microarray showing duplicate spots of recombinant ABLIM1 without (left) or with (right) recombinant DYRK1A, both with radioactive ATP. ( b ) Co-immunoprecipitation of exogenous DYRK1A and ABLIM1 proteins in Hek293T cells. Shown is a representative blot of three independent experiments. ( c ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM1. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( d ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM2. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( e ) Deconvolution images of F-actin fibres (phalloidin) in NIH3T3 cells transiently expressing the indicated constructs. Transfected cells were identified through co-transfection of nuclear H2B–GFP. The F-actin promoting Profilin (PFN1) was included as a positive control. Scale bar, 10 μm. ( f ) Quantification of the microscopy experiment depicted in e (mean of two independent experiments with at least 50 cells counted in each experiment). ARR, actin-rich region (lamellipodia, stress fibres, membrane ruffles or pronounced phalloidin positivity). ( g ) Subcellular localization of MKL1-GFP (green) in transiently transfected Hek293A cells. Representative pictures are shown. Nuclei appear in blue (4,6-diamidino-2-phenylindole). Scale bar, 10 μm. ( h ) Quantification of the experiment depicted in f . C, cytoplasmic; N, nuclear; N+C, nuclear and cytoplasmic localization of MKL1-GFP (mean of n =3 independent experiments. At least 100 cells were counted). ( i ) SRE (serum response element) luciferase reporter assay in Hek293T cells transiently transfected with the indicated constructs (mean of n =6±s.d.). ( j ) Phalloidin staining of siRNA-transfected NIH3T3 cells. Shown are representative images of two independent experiments. siABlim1/2: double knockdown of Ablim1 plus Ablim2 . Scale bar, 10 μm. ( k ) Phalloidin staining of siRNA/plasmid double-transfected NIH3T3 cells. The inset in the middle panel should help portrait the different phenotypes observed upon Ablim1 knockdown. siABlim1/2: double knockdown of Ablim1 plus Ablim2. Scale bar, 10 μm. * P <0.05; ** P <0.005; *** P <0.0005 (Student's t -test).

    Techniques Used: Autoradiography, Microarray, Recombinant, Immunoprecipitation, Fractionation, Stable Transfection, Expressing, Plasmid Preparation, Western Blot, Construct, Transfection, Cotransfection, Positive Control, Microscopy, Luciferase, Reporter Assay, Staining



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    protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit  (Thermo Fisher)
    90
    Thermo Fisher protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit
    ( a ) Autoradiograph of protein <t>microarray</t> showing duplicate spots of recombinant ABLIM1 without (left) or with (right) recombinant DYRK1A, both with radioactive ATP. ( b ) Co-immunoprecipitation of exogenous DYRK1A and ABLIM1 proteins in Hek293T cells. Shown is a representative blot of three independent experiments. ( c ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM1. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( d ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM2. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( e ) Deconvolution images of F-actin fibres (phalloidin) in NIH3T3 cells transiently expressing the indicated constructs. Transfected cells were identified through co-transfection of nuclear H2B–GFP. The F-actin promoting Profilin (PFN1) was included as a positive control. Scale bar, 10 μm. ( f ) Quantification of the microscopy experiment depicted in e (mean of two independent experiments with at least 50 cells counted in each experiment). ARR, actin-rich region (lamellipodia, stress fibres, membrane ruffles or pronounced phalloidin positivity). ( g ) Subcellular localization of MKL1-GFP (green) in transiently transfected Hek293A cells. Representative pictures are shown. Nuclei appear in blue (4,6-diamidino-2-phenylindole). Scale bar, 10 μm. ( h ) Quantification of the experiment depicted in f . C, cytoplasmic; N, nuclear; N+C, nuclear and cytoplasmic localization of MKL1-GFP (mean of n =3 independent experiments. At least 100 cells were counted). ( i ) SRE (serum response element) luciferase reporter assay in Hek293T cells transiently transfected with the indicated constructs (mean of n =6±s.d.). ( j ) Phalloidin staining of siRNA-transfected NIH3T3 cells. Shown are representative images of two independent experiments. siABlim1/2: double knockdown of Ablim1 plus Ablim2 . Scale bar, 10 μm. ( k ) Phalloidin staining of siRNA/plasmid double-transfected NIH3T3 cells. The inset in the middle panel should help portrait the different phenotypes observed upon Ablim1 knockdown. siABlim1/2: double knockdown of Ablim1 plus Ablim2. Scale bar, 10 μm. * P <0.05; ** P <0.005; *** P <0.0005 (Student's t -test).
    Protoarray Human Protein Microarray V5.0 Kinase Substrate Identification (Ksi) Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    protoarray human protein microarray v5.0 kinase substrate identification (ksi) kit - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Autoradiograph of protein microarray showing duplicate spots of recombinant ABLIM1 without (left) or with (right) recombinant DYRK1A, both with radioactive ATP. ( b ) Co-immunoprecipitation of exogenous DYRK1A and ABLIM1 proteins in Hek293T cells. Shown is a representative blot of three independent experiments. ( c ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM1. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( d ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM2. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( e ) Deconvolution images of F-actin fibres (phalloidin) in NIH3T3 cells transiently expressing the indicated constructs. Transfected cells were identified through co-transfection of nuclear H2B–GFP. The F-actin promoting Profilin (PFN1) was included as a positive control. Scale bar, 10 μm. ( f ) Quantification of the microscopy experiment depicted in e (mean of two independent experiments with at least 50 cells counted in each experiment). ARR, actin-rich region (lamellipodia, stress fibres, membrane ruffles or pronounced phalloidin positivity). ( g ) Subcellular localization of MKL1-GFP (green) in transiently transfected Hek293A cells. Representative pictures are shown. Nuclei appear in blue (4,6-diamidino-2-phenylindole). Scale bar, 10 μm. ( h ) Quantification of the experiment depicted in f . C, cytoplasmic; N, nuclear; N+C, nuclear and cytoplasmic localization of MKL1-GFP (mean of n =3 independent experiments. At least 100 cells were counted). ( i ) SRE (serum response element) luciferase reporter assay in Hek293T cells transiently transfected with the indicated constructs (mean of n =6±s.d.). ( j ) Phalloidin staining of siRNA-transfected NIH3T3 cells. Shown are representative images of two independent experiments. siABlim1/2: double knockdown of Ablim1 plus Ablim2 . Scale bar, 10 μm. ( k ) Phalloidin staining of siRNA/plasmid double-transfected NIH3T3 cells. The inset in the middle panel should help portrait the different phenotypes observed upon Ablim1 knockdown. siABlim1/2: double knockdown of Ablim1 plus Ablim2. Scale bar, 10 μm. * P <0.05; ** P <0.005; *** P <0.0005 (Student's t -test).

    Journal: Nature Communications

    Article Title: Identification of a novel actin-dependent signal transducing module allows for the targeted degradation of GLI1

    doi: 10.1038/ncomms9023

    Figure Lengend Snippet: ( a ) Autoradiograph of protein microarray showing duplicate spots of recombinant ABLIM1 without (left) or with (right) recombinant DYRK1A, both with radioactive ATP. ( b ) Co-immunoprecipitation of exogenous DYRK1A and ABLIM1 proteins in Hek293T cells. Shown is a representative blot of three independent experiments. ( c ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM1. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( d ) Cellular actin fractionation assay in NIH3T3 stably expressing empty vector control (mock) or ABLIM2. Jaspla, Jasplakinolide 10 nM for 30 min. Shown is a representative β-actin western blot of two independent experiments (the mean band intensity is given below). ( e ) Deconvolution images of F-actin fibres (phalloidin) in NIH3T3 cells transiently expressing the indicated constructs. Transfected cells were identified through co-transfection of nuclear H2B–GFP. The F-actin promoting Profilin (PFN1) was included as a positive control. Scale bar, 10 μm. ( f ) Quantification of the microscopy experiment depicted in e (mean of two independent experiments with at least 50 cells counted in each experiment). ARR, actin-rich region (lamellipodia, stress fibres, membrane ruffles or pronounced phalloidin positivity). ( g ) Subcellular localization of MKL1-GFP (green) in transiently transfected Hek293A cells. Representative pictures are shown. Nuclei appear in blue (4,6-diamidino-2-phenylindole). Scale bar, 10 μm. ( h ) Quantification of the experiment depicted in f . C, cytoplasmic; N, nuclear; N+C, nuclear and cytoplasmic localization of MKL1-GFP (mean of n =3 independent experiments. At least 100 cells were counted). ( i ) SRE (serum response element) luciferase reporter assay in Hek293T cells transiently transfected with the indicated constructs (mean of n =6±s.d.). ( j ) Phalloidin staining of siRNA-transfected NIH3T3 cells. Shown are representative images of two independent experiments. siABlim1/2: double knockdown of Ablim1 plus Ablim2 . Scale bar, 10 μm. ( k ) Phalloidin staining of siRNA/plasmid double-transfected NIH3T3 cells. The inset in the middle panel should help portrait the different phenotypes observed upon Ablim1 knockdown. siABlim1/2: double knockdown of Ablim1 plus Ablim2. Scale bar, 10 μm. * P <0.05; ** P <0.005; *** P <0.0005 (Student's t -test).

    Article Snippet: For the identification of DYRK1A protein substrates, we purchased the ProtoArray Human Protein Microarray v5.0 kinase substrate identification (KSI) kit from Invitrogen (#PAH0525065, containing ∼9,480 recombinant GST-tagged and baculovirus-expressed proteins in duplicate spots).

    Techniques: Autoradiography, Microarray, Recombinant, Immunoprecipitation, Fractionation, Stable Transfection, Expressing, Plasmid Preparation, Western Blot, Construct, Transfection, Cotransfection, Positive Control, Microscopy, Luciferase, Reporter Assay, Staining